Expression of tumor antigens on primary ovarian cancer cells compared to established ovarian cancer cell lines.

Zdroj: Oncotarget. 2016 Jul 19;7(29):46120-46126. doi: 10.18632/oncotarget.10028.
Autoři: K. Kloudová, H. Hromádková, S. Partlová, T. Brtnický, L. Rob, J. Bartůňková, M. Hensler, M.J. Halaška, R. Špíšek, A. Fialová

In order to select a suitable combination of cancer cell lines as an appropriate source of antigens for dendritic cell-based immunotherapy of ovarian cancer, we analyzed the expression level of 21 tumor associated antigens (BIRC5, CA125, CEA, DDX43, EPCAM, FOLR1, Her-2/neu, MAGE-A1, MAGE-A2, MAGE-A3, MAGE-A4, MAGE-A6, MAGE-A10, MAGE-A12, MUC-1, NY-ESO-1, PRAME, p53, TPBG, TRT, WT1) in 4 established ovarian cancer cell lines and in primary tumor cells isolated from the high-grade serous epithelial ovarian cancer tissue. More than 90% of tumor samples expressed very high levels of CA125, FOLR1, EPCAM and MUC-1 and elevated levels of Her-2/neu, similarly to OVCAR-3 cell line. The combination of OV-90 and OVCAR-3 cell lines showed the highest overlap with patients' samples in the TAA expression profile.